polyclonal rabbit anti wilms tumor protein  (Danaher Inc)

Journal: Frontiers in Oncology

Article Title: Primary extraovarian adult granulosa cell tumor of the greater omentum: a case report and literature review

doi: 10.3389/fonc.2025.1689815

Figure Lengend Snippet: Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) , WT-1 (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).

Article Snippet: WT-1 , Positive , Strong,diffuse nucleus , Supports diagnosis , OTIRIH(Rabbit Monoclonal), Zhongshan Golden Bridge.

Techniques: Staining, Immunohistochemical staining, Immunohistochemistry

Journal: Frontiers in Microbiology

Article Title: Roles of Sialic Acid, AXL, and MER Receptor Tyrosine Kinases in Mumps Virus Infection of Mouse Sertoli and Leydig Cells

doi: 10.3389/fmicb.2020.01292

Figure Lengend Snippet: Mumps virus (MuV) binding and internalization. (A) Cell purity. Sertoli cells (SC) and Leydig cells (LC) were isolated from three-week-old mice. Cell purity was assessed using immunofluorescence (IF) staining of Wilms tumor nuclear protein 1 (WT-1) for SC (upper panel) and 3β-HSD (lower panel) for LC. The cellular nuclei were counterstained with 4',6'-diamidino-2-phenylindole (DAPI). Insets in the upper right corners show the negative controls, in which pre-immune rabbit sera served as FIGURE 1the primary antibodies. (B,C) MuV binding. SC and LC were incubated with the indicated doses (MOI) of MuV on ice for 1 h. After washing twice with PBS, cells were treated with 0.25% trypsin (Try) for 5 min. Total RNA and protein were extracted. MuV nuclear protein (MuV-NP) RNA (B) and protein (C) levels were determined by real-time qRT-PCR and Western blot, respectively. (D,E) MuV internalization. SC and LC were incubated with 100 MOI of MuV at 37°C for 1 h: (D) Cells were treated with trypsin for 5 min. MuV-NP RNA (left panel) and protein (right panel) levels were determined by real-time qRT-PCR and Western blot, respectively. β-Actin was used as internal control for qRT-PCR and loading control for Western blot. (E) Intracellular MuV-NP were determined by IF staining with antibodies against MuV-NP (red). Cellular plasma and nuclei were visualized using IF for α-tubulin (green) and DAPI (blue) counterstaining, respectively. Cells, incubated with MuV-free PBS served as the controls (Ctrl). Images represent at least three independent experiments. Data are presented as the mean ± SEM of three experiments. Scale bars, 50 μm. ns, not significant.

Article Snippet: Monoclonal rat anti-MER (14-5751-82) antibody and Polyclonal rabbit anti-Wilms tumor nuclear protein 1 (WT1) antibody (PA5-16879) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Virus, Binding Assay, Isolation, Immunofluorescence, Staining, Wilms Tumor Assay, Incubation, Quantitative RT-PCR, Western Blot, Control, Clinical Proteomics